cd62l fitc mel-14 antibody Search Results


anti l selectin  (ATCC)
93
ATCC anti l selectin
Anti L Selectin, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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anti-mouse cd62l (mel-14  (Thermo Fisher)
90
Thermo Fisher anti-mouse cd62l (mel-14
Anti Mouse Cd62l (Mel 14, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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biotinylated anti-cd62 ligand (cd62l; mel-14  (Thermo Fisher)
90
Thermo Fisher biotinylated anti-cd62 ligand (cd62l; mel-14
Biotinylated Anti Cd62 Ligand (Cd62l; Mel 14, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
biotinylated anti-cd62 ligand (cd62l; mel-14 - by Bioz Stars, 2026-02
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dreg56  (ATCC)
93
ATCC dreg56
Dreg56, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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anti-ifn-γ-pe-cy7 (xmg1.2)  (Thermo Fisher)
90
Thermo Fisher anti-ifn-γ-pe-cy7 (xmg1.2)
Anti Ifn γ Pe Cy7 (Xmg1.2), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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cd25 antibody pc61.5  (Thermo Fisher)
90
Thermo Fisher cd25 antibody pc61.5
Cd25 Antibody Pc61.5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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cd5 53-7.3 antibody  (Thermo Fisher)
90
Thermo Fisher cd5 53-7.3 antibody
(A) Experimental protocol. Irradiated Rag2 -/- mice were reconstituted with bone marrow from OT-I WT TamCre or OT-I Srf f/f TamCre mice and the resulting chimeras fed with tamoxifen for 15 days. Purified OT-I WT (CD45.1) and OT-I Srf -/- (CD45.2) naïve CD8 + T-cells were then co-transferred 1:1 into CD45.1/CD45.2 recipients, and infected with rLM-OVA the following day. (B) Immunoblot analysis of SRF protein in Rag2 -/- reconstituted mice following 15 days tamoxifen treatment reveals complete Srf inactivation in OT-I Srf -/- cells. (C) Flow cytometry analysis following adoptive co-transfer of 5000 each OT-I WT and OT-I Srf -/- CD8 + T cells upon rLM-OVA infection. Data are mean numbers ± SEM in blood (20μl) or spleen, 3 mice per time point, and are representative of ≥3 independent experiments; nd, not detectable. (D) Cell surface expression of activation markers CD44 and <t>CD62L,</t> and MPEC/SLEC markers KLRG1 and IL-7Rα on OT-I WT TamCre (CD45.1 gated) and OT-I Srf -/- TamCre (CD45.2 gated) at day 8 post-infection in blood. Right panel, Proportions and numbers of SLEC (KLRG1 hi , IL-7Rα low ) and MPEC (KLRG1 low , IL-7Rα hi ) are shown. Data are means ± SEM; data points are individual mice, and are representative of ≥3 independent experiments. (E) CD8 + T cells were isolated 8 days post-infection, activated with 10nM SIINFEKL OVA peptide for 5h, and stained for IFN-γ, TNF-α and GranzB. Grey isotype control; black, experimental sample. Data are mean values ± SEM; data points represent individual mice.
Cd5 53 7.3 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cd5 53-7.3 antibody - by Bioz Stars, 2026-02
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fitc-conjugated anti-cd62l (mel-14) mabs  (Cedarlane)
90
Cedarlane fitc-conjugated anti-cd62l (mel-14) mabs
(A) Experimental protocol. Irradiated Rag2 -/- mice were reconstituted with bone marrow from OT-I WT TamCre or OT-I Srf f/f TamCre mice and the resulting chimeras fed with tamoxifen for 15 days. Purified OT-I WT (CD45.1) and OT-I Srf -/- (CD45.2) naïve CD8 + T-cells were then co-transferred 1:1 into CD45.1/CD45.2 recipients, and infected with rLM-OVA the following day. (B) Immunoblot analysis of SRF protein in Rag2 -/- reconstituted mice following 15 days tamoxifen treatment reveals complete Srf inactivation in OT-I Srf -/- cells. (C) Flow cytometry analysis following adoptive co-transfer of 5000 each OT-I WT and OT-I Srf -/- CD8 + T cells upon rLM-OVA infection. Data are mean numbers ± SEM in blood (20μl) or spleen, 3 mice per time point, and are representative of ≥3 independent experiments; nd, not detectable. (D) Cell surface expression of activation markers CD44 and <t>CD62L,</t> and MPEC/SLEC markers KLRG1 and IL-7Rα on OT-I WT TamCre (CD45.1 gated) and OT-I Srf -/- TamCre (CD45.2 gated) at day 8 post-infection in blood. Right panel, Proportions and numbers of SLEC (KLRG1 hi , IL-7Rα low ) and MPEC (KLRG1 low , IL-7Rα hi ) are shown. Data are means ± SEM; data points are individual mice, and are representative of ≥3 independent experiments. (E) CD8 + T cells were isolated 8 days post-infection, activated with 10nM SIINFEKL OVA peptide for 5h, and stained for IFN-γ, TNF-α and GranzB. Grey isotype control; black, experimental sample. Data are mean values ± SEM; data points represent individual mice.
Fitc Conjugated Anti Cd62l (Mel 14) Mabs, supplied by Cedarlane, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc-conjugated anti-cd62l (mel-14) mabs/product/Cedarlane
Average 90 stars, based on 1 article reviews
fitc-conjugated anti-cd62l (mel-14) mabs - by Bioz Stars, 2026-02
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cd122 biotin  (Thermo Fisher)
90
Thermo Fisher cd122 biotin
(A) Experimental protocol. Irradiated Rag2 -/- mice were reconstituted with bone marrow from OT-I WT TamCre or OT-I Srf f/f TamCre mice and the resulting chimeras fed with tamoxifen for 15 days. Purified OT-I WT (CD45.1) and OT-I Srf -/- (CD45.2) naïve CD8 + T-cells were then co-transferred 1:1 into CD45.1/CD45.2 recipients, and infected with rLM-OVA the following day. (B) Immunoblot analysis of SRF protein in Rag2 -/- reconstituted mice following 15 days tamoxifen treatment reveals complete Srf inactivation in OT-I Srf -/- cells. (C) Flow cytometry analysis following adoptive co-transfer of 5000 each OT-I WT and OT-I Srf -/- CD8 + T cells upon rLM-OVA infection. Data are mean numbers ± SEM in blood (20μl) or spleen, 3 mice per time point, and are representative of ≥3 independent experiments; nd, not detectable. (D) Cell surface expression of activation markers CD44 and <t>CD62L,</t> and MPEC/SLEC markers KLRG1 and IL-7Rα on OT-I WT TamCre (CD45.1 gated) and OT-I Srf -/- TamCre (CD45.2 gated) at day 8 post-infection in blood. Right panel, Proportions and numbers of SLEC (KLRG1 hi , IL-7Rα low ) and MPEC (KLRG1 low , IL-7Rα hi ) are shown. Data are means ± SEM; data points are individual mice, and are representative of ≥3 independent experiments. (E) CD8 + T cells were isolated 8 days post-infection, activated with 10nM SIINFEKL OVA peptide for 5h, and stained for IFN-γ, TNF-α and GranzB. Grey isotype control; black, experimental sample. Data are mean values ± SEM; data points represent individual mice.
Cd122 Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cd122 biotin - by Bioz Stars, 2026-02
90/100 stars
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l selectin mab lam 1 116  (Bio-Rad)
94
Bio-Rad l selectin mab lam 1 116
(A) Experimental protocol. Irradiated Rag2 -/- mice were reconstituted with bone marrow from OT-I WT TamCre or OT-I Srf f/f TamCre mice and the resulting chimeras fed with tamoxifen for 15 days. Purified OT-I WT (CD45.1) and OT-I Srf -/- (CD45.2) naïve CD8 + T-cells were then co-transferred 1:1 into CD45.1/CD45.2 recipients, and infected with rLM-OVA the following day. (B) Immunoblot analysis of SRF protein in Rag2 -/- reconstituted mice following 15 days tamoxifen treatment reveals complete Srf inactivation in OT-I Srf -/- cells. (C) Flow cytometry analysis following adoptive co-transfer of 5000 each OT-I WT and OT-I Srf -/- CD8 + T cells upon rLM-OVA infection. Data are mean numbers ± SEM in blood (20μl) or spleen, 3 mice per time point, and are representative of ≥3 independent experiments; nd, not detectable. (D) Cell surface expression of activation markers CD44 and <t>CD62L,</t> and MPEC/SLEC markers KLRG1 and IL-7Rα on OT-I WT TamCre (CD45.1 gated) and OT-I Srf -/- TamCre (CD45.2 gated) at day 8 post-infection in blood. Right panel, Proportions and numbers of SLEC (KLRG1 hi , IL-7Rα low ) and MPEC (KLRG1 low , IL-7Rα hi ) are shown. Data are means ± SEM; data points are individual mice, and are representative of ≥3 independent experiments. (E) CD8 + T cells were isolated 8 days post-infection, activated with 10nM SIINFEKL OVA peptide for 5h, and stained for IFN-γ, TNF-α and GranzB. Grey isotype control; black, experimental sample. Data are mean values ± SEM; data points represent individual mice.
L Selectin Mab Lam 1 116, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/l selectin mab lam 1 116/product/Bio-Rad
Average 94 stars, based on 1 article reviews
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fitc/percp-cy5.5 anti-cd62l mel-14  (Thermo Fisher)
90
Thermo Fisher fitc/percp-cy5.5 anti-cd62l mel-14
(A) Experimental protocol. Irradiated Rag2 -/- mice were reconstituted with bone marrow from OT-I WT TamCre or OT-I Srf f/f TamCre mice and the resulting chimeras fed with tamoxifen for 15 days. Purified OT-I WT (CD45.1) and OT-I Srf -/- (CD45.2) naïve CD8 + T-cells were then co-transferred 1:1 into CD45.1/CD45.2 recipients, and infected with rLM-OVA the following day. (B) Immunoblot analysis of SRF protein in Rag2 -/- reconstituted mice following 15 days tamoxifen treatment reveals complete Srf inactivation in OT-I Srf -/- cells. (C) Flow cytometry analysis following adoptive co-transfer of 5000 each OT-I WT and OT-I Srf -/- CD8 + T cells upon rLM-OVA infection. Data are mean numbers ± SEM in blood (20μl) or spleen, 3 mice per time point, and are representative of ≥3 independent experiments; nd, not detectable. (D) Cell surface expression of activation markers CD44 and <t>CD62L,</t> and MPEC/SLEC markers KLRG1 and IL-7Rα on OT-I WT TamCre (CD45.1 gated) and OT-I Srf -/- TamCre (CD45.2 gated) at day 8 post-infection in blood. Right panel, Proportions and numbers of SLEC (KLRG1 hi , IL-7Rα low ) and MPEC (KLRG1 low , IL-7Rα hi ) are shown. Data are means ± SEM; data points are individual mice, and are representative of ≥3 independent experiments. (E) CD8 + T cells were isolated 8 days post-infection, activated with 10nM SIINFEKL OVA peptide for 5h, and stained for IFN-γ, TNF-α and GranzB. Grey isotype control; black, experimental sample. Data are mean values ± SEM; data points represent individual mice.
Fitc/Percp Cy5.5 Anti Cd62l Mel 14, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fitc/percp-cy5.5 anti-cd62l mel-14/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
fitc/percp-cy5.5 anti-cd62l mel-14 - by Bioz Stars, 2026-02
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cd62l antibody  (Thermo Fisher)
90
Thermo Fisher cd62l antibody
(A) Experimental protocol. Irradiated Rag2 -/- mice were reconstituted with bone marrow from OT-I WT TamCre or OT-I Srf f/f TamCre mice and the resulting chimeras fed with tamoxifen for 15 days. Purified OT-I WT (CD45.1) and OT-I Srf -/- (CD45.2) naïve CD8 + T-cells were then co-transferred 1:1 into CD45.1/CD45.2 recipients, and infected with rLM-OVA the following day. (B) Immunoblot analysis of SRF protein in Rag2 -/- reconstituted mice following 15 days tamoxifen treatment reveals complete Srf inactivation in OT-I Srf -/- cells. (C) Flow cytometry analysis following adoptive co-transfer of 5000 each OT-I WT and OT-I Srf -/- CD8 + T cells upon rLM-OVA infection. Data are mean numbers ± SEM in blood (20μl) or spleen, 3 mice per time point, and are representative of ≥3 independent experiments; nd, not detectable. (D) Cell surface expression of activation markers CD44 and <t>CD62L,</t> and MPEC/SLEC markers KLRG1 and IL-7Rα on OT-I WT TamCre (CD45.1 gated) and OT-I Srf -/- TamCre (CD45.2 gated) at day 8 post-infection in blood. Right panel, Proportions and numbers of SLEC (KLRG1 hi , IL-7Rα low ) and MPEC (KLRG1 low , IL-7Rα hi ) are shown. Data are means ± SEM; data points are individual mice, and are representative of ≥3 independent experiments. (E) CD8 + T cells were isolated 8 days post-infection, activated with 10nM SIINFEKL OVA peptide for 5h, and stained for IFN-γ, TNF-α and GranzB. Grey isotype control; black, experimental sample. Data are mean values ± SEM; data points represent individual mice.
Cd62l Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd62l antibody/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
cd62l antibody - by Bioz Stars, 2026-02
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Image Search Results


(A) Experimental protocol. Irradiated Rag2 -/- mice were reconstituted with bone marrow from OT-I WT TamCre or OT-I Srf f/f TamCre mice and the resulting chimeras fed with tamoxifen for 15 days. Purified OT-I WT (CD45.1) and OT-I Srf -/- (CD45.2) naïve CD8 + T-cells were then co-transferred 1:1 into CD45.1/CD45.2 recipients, and infected with rLM-OVA the following day. (B) Immunoblot analysis of SRF protein in Rag2 -/- reconstituted mice following 15 days tamoxifen treatment reveals complete Srf inactivation in OT-I Srf -/- cells. (C) Flow cytometry analysis following adoptive co-transfer of 5000 each OT-I WT and OT-I Srf -/- CD8 + T cells upon rLM-OVA infection. Data are mean numbers ± SEM in blood (20μl) or spleen, 3 mice per time point, and are representative of ≥3 independent experiments; nd, not detectable. (D) Cell surface expression of activation markers CD44 and CD62L, and MPEC/SLEC markers KLRG1 and IL-7Rα on OT-I WT TamCre (CD45.1 gated) and OT-I Srf -/- TamCre (CD45.2 gated) at day 8 post-infection in blood. Right panel, Proportions and numbers of SLEC (KLRG1 hi , IL-7Rα low ) and MPEC (KLRG1 low , IL-7Rα hi ) are shown. Data are means ± SEM; data points are individual mice, and are representative of ≥3 independent experiments. (E) CD8 + T cells were isolated 8 days post-infection, activated with 10nM SIINFEKL OVA peptide for 5h, and stained for IFN-γ, TNF-α and GranzB. Grey isotype control; black, experimental sample. Data are mean values ± SEM; data points represent individual mice.

Journal: bioRxiv

Article Title: Defective cytoskeletal dynamics underlies the essential role of MRTF-SRF in IL-2 delivery to CD8 + T cells during infectious challenge

doi: 10.1101/2023.08.26.554941

Figure Lengend Snippet: (A) Experimental protocol. Irradiated Rag2 -/- mice were reconstituted with bone marrow from OT-I WT TamCre or OT-I Srf f/f TamCre mice and the resulting chimeras fed with tamoxifen for 15 days. Purified OT-I WT (CD45.1) and OT-I Srf -/- (CD45.2) naïve CD8 + T-cells were then co-transferred 1:1 into CD45.1/CD45.2 recipients, and infected with rLM-OVA the following day. (B) Immunoblot analysis of SRF protein in Rag2 -/- reconstituted mice following 15 days tamoxifen treatment reveals complete Srf inactivation in OT-I Srf -/- cells. (C) Flow cytometry analysis following adoptive co-transfer of 5000 each OT-I WT and OT-I Srf -/- CD8 + T cells upon rLM-OVA infection. Data are mean numbers ± SEM in blood (20μl) or spleen, 3 mice per time point, and are representative of ≥3 independent experiments; nd, not detectable. (D) Cell surface expression of activation markers CD44 and CD62L, and MPEC/SLEC markers KLRG1 and IL-7Rα on OT-I WT TamCre (CD45.1 gated) and OT-I Srf -/- TamCre (CD45.2 gated) at day 8 post-infection in blood. Right panel, Proportions and numbers of SLEC (KLRG1 hi , IL-7Rα low ) and MPEC (KLRG1 low , IL-7Rα hi ) are shown. Data are means ± SEM; data points are individual mice, and are representative of ≥3 independent experiments. (E) CD8 + T cells were isolated 8 days post-infection, activated with 10nM SIINFEKL OVA peptide for 5h, and stained for IFN-γ, TNF-α and GranzB. Grey isotype control; black, experimental sample. Data are mean values ± SEM; data points represent individual mice.

Article Snippet: Antibodies were from: eBioscience: CD8 (53-6.7), CD122 (5H4),TCRβ (H57-597), IRF4 (3E4), IFNγ (XMG1.2), CD44 (IM7), CD122 (5H4), CD5(53-7.3) CD62L (MEL-14), CXCR3 (173), CCR7 (4B12), NKG2D (CX5), TNF-α (MP6-XT22), GranzymeB (NGZB), IRF4(3E4).

Techniques: Irradiation, Purification, Infection, Western Blot, Flow Cytometry, Expressing, Activation Assay, Isolation, Staining, Control

(A) Time course (days) of SRF expression in tamoxifen-fed animals. (B) OT-I WT and OT-I Srf -/- CD8 + T cells were MACS-purified from the spleens of tamoxifen-treated bone marrow reconstituted mice and stained for TCRβ, CD8, CD5, CCR7, CD62L and CD44 expression. (C) Purified OT-I cells were stimulated for 5h with SIINFEKL OVA peptide and analysed for intracellular IFN-γ and TNF-α expression. (D) IL-7Rα, CD62L and CD44 expression in vivo after LM-OVA infection in spleen and blood.

Journal: bioRxiv

Article Title: Defective cytoskeletal dynamics underlies the essential role of MRTF-SRF in IL-2 delivery to CD8 + T cells during infectious challenge

doi: 10.1101/2023.08.26.554941

Figure Lengend Snippet: (A) Time course (days) of SRF expression in tamoxifen-fed animals. (B) OT-I WT and OT-I Srf -/- CD8 + T cells were MACS-purified from the spleens of tamoxifen-treated bone marrow reconstituted mice and stained for TCRβ, CD8, CD5, CCR7, CD62L and CD44 expression. (C) Purified OT-I cells were stimulated for 5h with SIINFEKL OVA peptide and analysed for intracellular IFN-γ and TNF-α expression. (D) IL-7Rα, CD62L and CD44 expression in vivo after LM-OVA infection in spleen and blood.

Article Snippet: Antibodies were from: eBioscience: CD8 (53-6.7), CD122 (5H4),TCRβ (H57-597), IRF4 (3E4), IFNγ (XMG1.2), CD44 (IM7), CD122 (5H4), CD5(53-7.3) CD62L (MEL-14), CXCR3 (173), CCR7 (4B12), NKG2D (CX5), TNF-α (MP6-XT22), GranzymeB (NGZB), IRF4(3E4).

Techniques: Expressing, Purification, Staining, In Vivo, Infection

(A) MPEC and SLEC generation in infected splenocytes. Wildtype cells were co-transferred with cells in which Srf was inactivated either prior to transfer (OT-I Srf -/- (pre)) or after transfer (OT-I Srf -/- (post)), and infected as outlined in (OT-I Srf -/- (pre)), or (OT-I Srf -/- (post)). Data show mean values ± SEM; data points represent individual mice; n=6. (B) Cytokine production by infected splenocytes stimulated with SIINFEKL peptide (OVA; 10nM) for 5 hours. Wildtype cells were co-transferred with cells in which Srf was inactivated either prior to transfer (OT-I Srf -/- (pre)) or following transfer and infection (OT-I Srf -/- (post)). Data show mean values ± SEM; data points represent individual mice. (C) Memory-like OT-I WT and Srf -/- (post) cells, defined by CD62L, CCR7 and IL-2Rβ expression. (D) Srf mRNA levels in sorted OT-I splenocytes at day 50 post-infection.

Journal: bioRxiv

Article Title: Defective cytoskeletal dynamics underlies the essential role of MRTF-SRF in IL-2 delivery to CD8 + T cells during infectious challenge

doi: 10.1101/2023.08.26.554941

Figure Lengend Snippet: (A) MPEC and SLEC generation in infected splenocytes. Wildtype cells were co-transferred with cells in which Srf was inactivated either prior to transfer (OT-I Srf -/- (pre)) or after transfer (OT-I Srf -/- (post)), and infected as outlined in (OT-I Srf -/- (pre)), or (OT-I Srf -/- (post)). Data show mean values ± SEM; data points represent individual mice; n=6. (B) Cytokine production by infected splenocytes stimulated with SIINFEKL peptide (OVA; 10nM) for 5 hours. Wildtype cells were co-transferred with cells in which Srf was inactivated either prior to transfer (OT-I Srf -/- (pre)) or following transfer and infection (OT-I Srf -/- (post)). Data show mean values ± SEM; data points represent individual mice. (C) Memory-like OT-I WT and Srf -/- (post) cells, defined by CD62L, CCR7 and IL-2Rβ expression. (D) Srf mRNA levels in sorted OT-I splenocytes at day 50 post-infection.

Article Snippet: Antibodies were from: eBioscience: CD8 (53-6.7), CD122 (5H4),TCRβ (H57-597), IRF4 (3E4), IFNγ (XMG1.2), CD44 (IM7), CD122 (5H4), CD5(53-7.3) CD62L (MEL-14), CXCR3 (173), CCR7 (4B12), NKG2D (CX5), TNF-α (MP6-XT22), GranzymeB (NGZB), IRF4(3E4).

Techniques: Infection, Expressing

(A) Experimental protocol for analysis of OT-I Elk4 -/- CD8 + T cells. (B) Analysis by flow cytometry of expansion of 5000 adoptively co-transferred OT-I Elk4 -/- and OT-I WT CD8 + cells after infection, mean value ± SEM (n=6 mice). (C) Top, representative profile of cell surface expression of KLRG1 and IL-7Rα, 7 days post-infection; bottom, quantification of SLEC and MPEC. Data are mean values ± SEM; data points represent individual mice. (D) Irradiated Rag2 -/- mice were reconstituted with bone marrow from OT-I WT TamCre or OT-I Mrtfa - /- Mrtfb f/f TamCre mice. Following reconstitution, chimeras were treated for 15 days with tamoxifen and OT-I WT (CD45.1) and OT-I Mrtfab -/- (CD45.2) naïve CD8 + T cells were purified and co-transferred 1:1 into CD45.1/CD45.2 recipients, followed by rLM-OVA infection the next day. (E) Immunoblot analysis before transfer reveals complete Mrtfb inactivation. (F) Analysis by flow cytometry of expansion of 5000 adoptively co-transferred OT-I WT and OT-I Mrtfab -/- CD8 + T cells following infection. Data show mean numbers ± SEM in the blood (20μl). Data are representative of 2 independent experiments with 5 mice each. (G) Cell surface expression of CD44, CD62L, KLRG1 and IL-7Rα by flow cytometry as in . Quantification of SLEC and MPEC. Data show mean values ± SEM; data points represent individual mice pooled from 2 independent experiments with 6 mice each.

Journal: bioRxiv

Article Title: Defective cytoskeletal dynamics underlies the essential role of MRTF-SRF in IL-2 delivery to CD8 + T cells during infectious challenge

doi: 10.1101/2023.08.26.554941

Figure Lengend Snippet: (A) Experimental protocol for analysis of OT-I Elk4 -/- CD8 + T cells. (B) Analysis by flow cytometry of expansion of 5000 adoptively co-transferred OT-I Elk4 -/- and OT-I WT CD8 + cells after infection, mean value ± SEM (n=6 mice). (C) Top, representative profile of cell surface expression of KLRG1 and IL-7Rα, 7 days post-infection; bottom, quantification of SLEC and MPEC. Data are mean values ± SEM; data points represent individual mice. (D) Irradiated Rag2 -/- mice were reconstituted with bone marrow from OT-I WT TamCre or OT-I Mrtfa - /- Mrtfb f/f TamCre mice. Following reconstitution, chimeras were treated for 15 days with tamoxifen and OT-I WT (CD45.1) and OT-I Mrtfab -/- (CD45.2) naïve CD8 + T cells were purified and co-transferred 1:1 into CD45.1/CD45.2 recipients, followed by rLM-OVA infection the next day. (E) Immunoblot analysis before transfer reveals complete Mrtfb inactivation. (F) Analysis by flow cytometry of expansion of 5000 adoptively co-transferred OT-I WT and OT-I Mrtfab -/- CD8 + T cells following infection. Data show mean numbers ± SEM in the blood (20μl). Data are representative of 2 independent experiments with 5 mice each. (G) Cell surface expression of CD44, CD62L, KLRG1 and IL-7Rα by flow cytometry as in . Quantification of SLEC and MPEC. Data show mean values ± SEM; data points represent individual mice pooled from 2 independent experiments with 6 mice each.

Article Snippet: Antibodies were from: eBioscience: CD8 (53-6.7), CD122 (5H4),TCRβ (H57-597), IRF4 (3E4), IFNγ (XMG1.2), CD44 (IM7), CD122 (5H4), CD5(53-7.3) CD62L (MEL-14), CXCR3 (173), CCR7 (4B12), NKG2D (CX5), TNF-α (MP6-XT22), GranzymeB (NGZB), IRF4(3E4).

Techniques: Flow Cytometry, Infection, Expressing, Irradiation, Purification, Western Blot

(A) OT-I WT or OT-I Mrtfab - /- cells were MACS-purified from the spleens of tamoxifen-treated bone marrow reconstituted mice and stained for TCRβ, CD62L and CD44 expression. (B) Expression of IL-7Rα, CD62L and CD44 on OT-I WT or OT-I Mrtfab - /- CD8 + T cells following LM-OVA infection in blood and spleen.

Journal: bioRxiv

Article Title: Defective cytoskeletal dynamics underlies the essential role of MRTF-SRF in IL-2 delivery to CD8 + T cells during infectious challenge

doi: 10.1101/2023.08.26.554941

Figure Lengend Snippet: (A) OT-I WT or OT-I Mrtfab - /- cells were MACS-purified from the spleens of tamoxifen-treated bone marrow reconstituted mice and stained for TCRβ, CD62L and CD44 expression. (B) Expression of IL-7Rα, CD62L and CD44 on OT-I WT or OT-I Mrtfab - /- CD8 + T cells following LM-OVA infection in blood and spleen.

Article Snippet: Antibodies were from: eBioscience: CD8 (53-6.7), CD122 (5H4),TCRβ (H57-597), IRF4 (3E4), IFNγ (XMG1.2), CD44 (IM7), CD122 (5H4), CD5(53-7.3) CD62L (MEL-14), CXCR3 (173), CCR7 (4B12), NKG2D (CX5), TNF-α (MP6-XT22), GranzymeB (NGZB), IRF4(3E4).

Techniques: Purification, Staining, Expressing, Infection